Primary culture of intestinal epithelial cells as a potential model for Toxoplasma gondii enteric cycle studies

Mem Inst Oswaldo Cruz. 2009 Sep;104(6):862-4.

Primary culture of intestinal epithelial cells as a potential model for Toxoplasma gondii enteric cycle studies

Moura Mde A, Amendoeira MR, Barbosa HS.

Laboratório de Toxoplasmose, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil, 21040-361.

The primary culture of intestinal epithelial cells from domestic cats is an efficient cellular model to study the enteric cycle of Toxoplasma gondii in a definitive host. The parasite-host cell ratio can be pointed out as a decisive factor that determines the intracellular fate of bradyzoites forms. The development of the syncytial-like forms of T. gondii was observed using the 1:20 bradyzoite-host cell ratio, resulting in similar forms described in in vivo systems. This alternative study potentially opens up the field for investigation into the molecular aspects of this interaction. This can contribute to the development of new strategies for intervention of a main route by which toxoplasmosis spreads.

PMID: 19876557 [PubMed - in process]