Early detection of Toxoplasma gondii-infected cats by interferon-gamma release assay

Exp Parasitol. 2015 Aug 19. pii: S0014-4894(15)30032-1. doi: 10.1016/j.exppara.2015.08.015. [Epub ahead of print]

Yin Q1, El-Ashram S2, Liu XY3, Suo X4.

Author information

Abstract

Felines, the only definitive hosts that shed the environmentally-durable oocysts, are the key in the transmission of Toxoplasma gondii to all warm-blooded animals. They seroconvert as late as the third week and begin to shed oocysts as early as 3-8 days after being fed tissue cysts. Early detection of Toxoplasma-infected cats is crucial to evaluate Toxoplasma-contaminated environment and potential risks to public health. Moreover, it is fundamental for Toxoplasma infection control. Interferon-gamma release assay (IGRA) is a blood-based test assessing the presence of IFN-γ released by the T-lymphocytes directed against specific antigens, which is an ideal assay for early detection of Toxoplasma-infected cats. Here, cats were orally infected with the tissue cysts and blood was collected for toxoplasmic antigen stimulation, and the released IFN-γ was measured by ELISA. Results showed that Toxoplasma-infection was detected by IGRA as early as 4 days post-infection (dpi); while serum Toxoplasma IgM and IgG were detected by ELISA at 10 dpi and 14 dpi, respectively. Our findings demonstrated that IGRA-positive and ELISA-negative samples revealed an early Toxoplasma infection in cats, indicating a new strategy for the early diagnosis of Toxoplasma infection by combining IGRA and ELISA. Therefore, IGRA could emerge as a reliable diagnostic tool for the exploration of cat toxoplasmosis prevalence and its potential risks to public health.

Copyright © 2015. Published by Elsevier Inc.

KEYWORDS: 

Toxoplasma gondii; cat; early detection; interferon-gamma release assay

PMID:

 

26297953

 

[PubMed - as supplied by publisher]